Establishment of BALB/c Mouse Infected with Frozen <i>Helicobacter pylori</i>

Original Article

The Korean Journal of Helicobacter and Upper Gastrointestinal Research 2009;9(2):82-89.
Published online December 30, 2009.

Establishment of BALB/c Mouse Infected with Frozen Helicobacter pylori

Frozen Helicobacter pylori 감염시킨 BALB/c 마우스 모델 수립

송인도ㆍ김범진ㆍ오형철ㆍ김정욱ㆍ김재규

중앙대학교 의과대학 내과학교실

Abstract

Background/Aims
To understand the pathogenesis of H. pylori infection, an ideal animal model to be cost effective, easy to work with and reproduce the various aspects of H. pylori disease is required. The aim of this study was to establish BALB/c mouse infected with frozen H. pylori. Methods: BALB/c mice were inoculated intragastrically with 2×10⁹ CFU of SS1 and HP99, the mouse adapted H. pylori. Loss of glandular architecture, erosions and infiltration of inflammatory cells with normal gastric mucosa were investigated. In the 4, 6 and 8 weeks after bacterial inoculation, H. pylori infected mice were sacrificed. Evidence for H. pylori infection was assessed by CLO test, Hematoxylin & Eosin stain and by UreC PCR of gastric mucosa. Results: The H. pylori colonization and positive CLO tests were observed in all inoculated mice. The UreC PCR showed that the inoculated mouse isolate gave an identical band pattern to the original frozen H. pylori strains. No definite infiltration of inflammatory cell and gastric mucosal atrophy were shown. Conclusions: The additional study of BALB/c mouse model infected with fresh and frozen H. pylori strains (SS1, HP99) more than 8 weeks is needed. (The Korean Journal of Helicobacter and Upper Gastrointestinal Research 2009;9:82-89)

Key Words: Helicobacter pylori, Animal model, BALB/c mouse