This study provides an important contribution to the ongoing efforts in diagnosing H. pylori infection and identifying clarithromycin resistance using molecular techniques [6]. Given the increasing prevalence of clarithromycin-resistant H. pylori strains, rapid and accurate diagnostic tools are essential for guiding effective eradication therapies. This study evaluates two real-time polymerase chain reaction assays—Allplex^TM H. pylori & ClariR Assay and Ezplex^® HP-CLA—comparing their performance against traditional diagnostic methods (rapid urease test) and H. pylori culture and antibiotic susceptibility test [6]. Molecular diagnostics have gained prominence over culture-based methods due to their efficiency and reliability in detecting resistance-associated mutations [7]. The findings demonstrate that both AllplexTM and Ezplex^® exhibit high diagnostic accuracy, with Ezplex^® achieving a remarkable eradication rate of 90.7% when used to guide tailored therapy. The study also highlights the clinical relevance of genotypic resistance detection in optimizing treatment strategies, potentially improving patient outcomes and reducing antibiotic misuse [6].

Overall, this study is a valuable addition to the field, reinforcing the importance of molecular testing in H. pylori management [6]. Clinicians and microbiologists should consider integrating these advanced diagnostic tools into routine practice to enhance treatment efficacy and combat antibiotic resistance.