Autoimmune Gastritis Accompanied by a Schwannoma Presenting as a Subepithelial Tumor

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Korean J Helicobacter Up Gastrointest Res. 2023;23(2):137-142

Publication date (electronic) : 2023 June 12

doi : https://doi.org/10.7704/kjhugr.2023.0021

Yong Hwan Ahn¹, Kyo Bum Hwang¹, Geom Seog Seo^,2

¹Department of Internal Medicine, Plus I-miko Hospital, Suncheon, Korea

²Department of Internal Medicine, Digestive Disease Research Institute, Wonkwang University School of Medicine, Iksan, Korea

Corresponding author: Geom Seog Seo Department of Internal Medicine, Digestive Disease Research Institute, Wonkwang University School of Medicine, 460 Iksan-daero, Iksan 54538, Korea Tel: +82-63-859-2565, Fax: +82-63-855-2025, E-mail: medsgs@wonkwang.ac.kr

Received 2023 February 17; Revised 2023 March 13; Accepted 2023 March 26.

Abstract

INTRODUCTION

During embryonic development, the stomach develops from a gastric pouch divided into 2 anatomical and functional parts: the oxyntic mucosa, which forms the gastric body and fundus, and the mucous-secreting mucosa, which forms the antrum [1]. Atrophic gastritis is caused by chronic infiltration of inflammatory cells into the lamina propria, which destroys the mucosal glands, decreasing mucosal secretion in the stomach. The two most common causes are Helicobacter pylori infection and autoimmunity [1-3]. Autoimmune gastritis (AIG) is caused by an auto‑antibody targeting of the parietal cell’s H⁺/K⁺ ATPase. These complexes periodically destroy parietal cells via an auto-reactivated CD4⁺ T-cell-mediated immune response and activation of cytotoxic T cells. AIG leads to widespread atrophy of the gastric body and fundus[3]. Continuous destruction of parietal cells decreases secretion of gastric acid and intrinsic factors, which causes increased gastrin secretion from G cells in the antrum. This leads to hyperplasia of enterochromaffin-like (ECL) cells that secrete histamine in the gastric body or fundus, which further leads to neuroendocrine tumor (NET) development. ECL cells are located in the deep oxyntic mucosal area in patients with AIG; similarly to subepithelial tumors (SET) of NETs [3,4]. Although EUS is the best method to distinguish SETs and NETs, tissue biopsy may be required for a precise diagnosis, as well as additional endoscopic or surgical resection. Tumor resection or biopsy is often recommended for SETs measuring >2 cm. However, endoscopic resection is considered for tumors <2 cm classified as NETs or tumors with uncertain histological characteristics [5]. The occurrence of SETs in AIG accounts for 5% of neoplastic diseases occurring in the gastric mucosa and management has not yet been established [6]. Here, we report the case of a patient diagnosed with AIG after a serologic test, endoscopy, and biopsy of a <2 cm fixed-form SET in the lower body of the stomach that required wedge resection for accurate diagnosis and treatment, which confirmed it to be schwannoma. This case was approved by the Institutional Review Board of Wonkwang university hospital (No. WKUH 2023-03-012). Informed consent for it was obtained from patient for publication of this study.

CASE REPORT

A 54-year-old female patient presented at our hospital with a chief complaint of indigestion after meals for 2 years intermittently. At the time, the patient had chronic gastritis resulting from a previous hospital endoscopy, but had not undergone further testing and no previous endoscopic images were available. The patient was not treated for H. pylori infection, did not take any medications, including antacids and antibiotics, and had no underlying diseases for which she was undergoing regular follow-up examinations. We performed an upper gastrointestinal (GI) endoscopy, which revealed erythematous nodules, mucosal fold flattening, and atrophy on the greater curvature of the gastric body. However, there was no significant atrophy at the antrum area (Fig. 1). Serologic test results were pepsinogen Ⅰ level, 22.4 ng/mL; pepsinogen Ⅱ level, 13.2 ng/mL; and pepsinogen Ⅰ/Ⅱ ratio, 1.7. We found that serum gastrin level was high (205 pg/mL; reference value: 13~115 pg/mL), and that the anti-Helicobacter pylori antibody level was normal (0.73 U/mL; reference value: 0.0~0.89 U/mL). Upper GI endoscopy revealed atrophic gastritis in the gastric body and fundus, and a rapid urease test for H. pylori infection was negative. Hence, immunofluorescence analysis for anti-parietal cell antibodies was performed (Kallestad Mouse Stomach/Kidney; Bio-RAD, Hercules, CA, USA), which was positive. Biopsies were collected from the greater curvature of the gastric body and antrum. Notably, biopsy was performed on the slightly elevated erythematous nodule and the atrophic lesion at the greater curvature of the gastric body (Fig. 2A). From the antrum biopsy findings, the mucosa was found to be normal with no significant infiltration of inflammatory cells. However, biopsy performed in the body area showed inflammatory cell infiltration in the mucosal layer, confirming the AIG diagnosis. Inflammatory cell infiltration was significantly higher in the atrophic lesion than erythematous nodular lesion in the gastric body (Fig. 2B, C). Immunohistochemistry with chromogranin A was performed to compare ECL cells in the erythematous nodule area and the atrophic lesion in the gastric body; atrophic lesion had increased linear ECL cell hyperplasia compared to the erythematous nodular lesion (Fig. 2D, E). Upper GI endoscopy revealed a SET located at the greater curvature of the gastric lower body. The tumor measured slightly larger than 1 cm, did not show any rolling or cushion signs, and had a fixed shape (Fig. 3A). An abdominal computed tomography scan was performed to evaluate the SET size and location, which measured 1.2 cm and showed exophytic lesion in the gastric body (Fig. 3B). EUS showed a 1.1×0.8 cm oval tumor beneath the muscularis propria (Fig. 3C). The patient wanted the lesion excised and a wedge resection was performed for treatment and biopsy. The tumor’s dimensions and weight were 1.5×1 cm and 6 g, respectively, and it had a smooth surface. The inner surface of the tumor was gray, and biopsy findings revealed interlacing bundles of spindle cells (Fig. 3D). Simultaneously, immunohistochemical staining for S-100 protein was performed, and schwannoma was diagnosed with the positive findings (Fig. 3E). One month postoperatively, the patient no longer complained of GI symptoms.

Fig. 1.

Upper gastrointestinal endoscopy findings. (A) Erythematous nodules, flattened mucosal folds, and atrophic mucosa are observed in the gastric body and fundus. (B) Flattened gastric mucosal folds and prominent submucosal vasculature are observed along the greater curvature of the gastric body. (C) The gastric antrum appears normal.

Fig. 2.

Histopathological findings of the greater curvature of the gastric body. (A) Endoscopic images showing slightly elevated erythematous nodules (blue arrow) and advanced mucosal atrophy (yellow arrow) along the greater curvature of the gastric mid body. (B) Specimen obtained from an erythematous nodule showing mild inflammation and atrophic oxyntic gastric mucosa (H&E stain, ×100). (C) Specimen obtained from the mucosa in an area of advanced atrophy showing moderate inflammation and atrophic oxyntic gastric mucosa (H&E stain, ×100). (D) Image of the same specimen as that shown in (B); mildly increased numbers of neuroendocrine cells are observed (chromogranin A stain, ×100). (E) Image of the same specimen as that shown in (C); a mild-to-moderate increase in the numbers of neuroendocrine cells and linear neuroendocrine cell hyperplasia is visualized (chromogranin A stain, ×100).

Fig. 3.

Images of the subepithelial tumor. (A) Endoscopic images showing a subepithelial tumor (slightly >1 cm) at the greater curvature of the gastric lower body. (B) Contrast-enhanced computed tomography scan showing an enhanced exophytic mass (1.2 cm) in the gastric body. (C) Endoscopic ultrasonography scan showing an oval hypoechoic mass (1.1×0.8 cm) with a clearly demarcated border, beneath the muscularis propria layer. (D) Specimen showing interlacing bundles of spindle cells with elongated and palisading nuclei (hematoxylin & eosin stain, ×100). (E) Specimen showing stained cytoplasm and nuclei of tumor cells (S-100 protein stain, ×100).

DISCUSSION

AIG is characterized by atrophic changes in the gastric body and a normal mucous membrane in the antrum. However, the remaining parietal cells can appear as various oxyntic mucosal lesions in the gastric body, such as flat, elevated, pseudopolypoid, island, extensive, and granular lesions. Pathological changes can lead to H. pylori infection or bile acid regurgitation in the gastric antrum, such as patchy redness, circular wrinkle-like pattern, and red streak; likely to be missed on upper GI endoscopies. Mucosal changes may not be obvious in early stages of AIG, making a definitive diagnosis difficult unless gastric body and antrum biopsies and a serologic test are performed [7]. Slightly elevated erythematous nodules found in the gastric body on an upper GI endoscopy may be a characteristic feature of early-stage AIG. When erythematous and atrophic mucosa biopsies are performed, damage to the parietal and chief cells is greater in the atrophic mucosa than in the erythematous mucosa [8]. In the present case, erythematous nodules and atrophic mucosa were observed together in the gastric body, but inflammatory cell infiltration and ECL cell hyperplasia were more severe in the atrophic mucosa than in erythematous nodules. Serologic testing revealed low pepsinogen Ⅰ and pepsinogen Ⅰ/Ⅱ ratio and high serum gastrin levels, however pepsinogen I was higher and gastrin levels were lower than those observed in AIG patients [9]. Thus, this case is an example of an ongoing AIG stage, not early or advanced atrophy stage. AIG is a progressive inflammatory disease that can appear as various features on endoscopy depending on its stage, and show ambiguous serologic results, thus biopsy should be performed even if widespread atrophy is not apparent, and remnant oxyntic mucosal lesions mixed with atrophic mucosa and flattened folds are observed in the gastric body [10].

Asymptomatic gastric SETs <2 cm without ulceration, bleeding, or with increasing size can be followed up regularly by EUS. However, biopsy should not be avoided solely based on tumor size but should be considered when the mass arises from the muscularis propria or suspected of malignancy [11]. ECL cells are located deeper in the mucosa and infiltrate the submucosal layer in AIG. Although NETs from ECL cells did not occur in the proper muscle layer in this patient, they may resemble SETs, and endoscopic resection is recommended even if they are <1 cm to avoid the risk of lymph node metastasis [12]. Therefore, NETs accompanied by AIG may appear as SETs, occur in various locations, and may hide malignancy when they are <2 cm [13-17]. Table 1 summarizes cases reporting SETs in AIG patients thus far. A schwannoma, confirmed by biopsy, is a benign, asymptomatic tumor that can be discovered incidentally and has a good prognosis. Endoscopic resection is effective in some cases; however, it is not easy to perform. Hence, surgical resection remains the primary treatment option [18]. In the present case, the SET measured <2 cm, and EUS and computed tomography confirmed that the tumor, which was accompanied by AIG, was fixed, and located under the muscularis propria. Even though 60% of tumors originating from the proper muscle layer are GI stromal tumors, the EUS in this case showed a hypoechoic and homogenous tumor, consistent with a potential schwannoma diagnosis [5]. In the case of SETs <2 cm originating from the proper muscle layer, mucosal incision-assisted biopsy, EUS guided-fine needle biopsy or tumor resection to avoid unnecessary follow up may be an option in case of unknown histology [19]. Moreover, as the patient wanted the tumor excised, a wedge resection was performed to facilitate treatment and establish an accurate histological differential diagnosis of SETs originating from the muscularis propria such as schwannoma, leiomyoma or GI stromal tumor [5,19].

Table 1.

Reported Cases of Autoimmune Gastritis with Concomitant Subepithelial Tumors

Hypergastrinemia resulting from the hypochlorhydria environment may contribute to ECL cell proliferation from hyperplasia to NETs [20]. In our case, as shown in Table 1, gastrin levels were not high compared to other cases; however, it is not yet known what effect hypergastrinemia has on SET development in AIG. There are no definitive guidelines for assessment and treatment of SETs in patients with AIG. Moreover, the correlation between these tumors and AIG is quite ambiguous; a schwannoma found in a SET may not be related to AIG. As SETs can manifest in various forms in patients with AIG, a systematic follow-up of similar cases, such as this one, is required.

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