Relation between Toll Like Receptor-2 Polymorphism and <i>Helicobacter pylori </i>Associated Peptic Ulcer Disease

Original

The Korean Journal of Helicobacter and Upper Gastrointestinal Research 2008;8(1):20-25.
Published online July 10, 2008.

Relation between Toll Like Receptor-2 Polymorphism and Helicobacter pylori Associated Peptic Ulcer Disease

Helicobacter pylori 감염에 의한 소화성 궤양과 Toll Like Receptor-2 유전자 다형성과의 관계

김형수ㆍ신운건ㆍ김경호ㆍ장명국ㆍ박준용ㆍ이진헌ㆍ김하정*ㆍ김학양

한림대학교 의과대학 내과학교실, 소화기연구실*

Abstract

Background/Aims
Helicobacter pylori (H. pylori) infection is one of the most important causes of peptic ulcer disease. Recently, Toll-like receptor (TLR) 2, but not TLR4 is required for H. pylori lipopolysaccharide induced nuclear factor-kappa B activation. Therefore, it is important to investigate the relation between TLR2 polymorphism and peptic ulcer disease for the understanding of peptic ulcer disease pathophysiology. This study aimed to investigate the relation between the TLR2 polymorphism and the incidence of peptic ulcer disease in H. pylori infected Korean patients. Methods: Blood samples were collected from a total of 190 H. pylori infected patients (gastric ulcer; n=74, duodenal ulcer; n=68 and control; n=48). DNA was extracted from buffy coat by using commercially available extraction kit. TLR2 Arg753Gln polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: Although we found TLR2 PCR product in all cases, TLR2 Arg753Gln polymorphism was not detected in this study subjects. Conclusions: In conclusion, the polymorphism of TLR2 (TLR2 Arg753Gln) does not seem to be associated with pathogenesis of H. pylori related peptic ulcer disease in Korean patients. (The Korean Journal of Helicobacter and Upper Gastrointestinal Research 2008;8:20-25)

Key Words: Toll-like receptor, Polymorphism, Helicobacter pylori, Peptic ulcer disease