<em>Helicobacter pylori</em> Induces the Change of Surface Microprocesses and Cytoskeleton in AGS and Hep-G2 Cell Lines

Original

대한Helicobacter 연구학회지 2002;2(1):1-9.
Published online June 21, 2002.

Helicobacter pylori Induces the Change of Surface Microprocesses and Cytoskeleton in AGS and Hep-G2 Cell Lines

Helicobacter pylori 유착에 의한 AGS 및 Hep-G2 세포 표면 구조 및 세포골격 변화에 대한 전자현미경적 연구

이정환*·전훈재·정록선·김경오·김용식·김영선·박철희·진윤태·이상우·최재현·이홍식·김창덕·류호상·문정섭*·박은경^†·안소현^†·엄창섭^†·현진해

고려대학교 의과대학 내과학교실, 소화기연구소, ^†유전병연구소, *인제대학교 의과대학 내과학교실

Abstract

Background/Aims: The attachment of Helicobacter pylori(H. pylori) to the gastric epithelial cells is a prerequisite for the induction of various gastrointestinal diseases. Except for the morphological patterns, exact nature and mechanism of H. pylori attachment to the gastric epithelial cells have not been defined well. This study was designed to elucidate the direct interaction of H. pylori with gastric epithelial cells using in vitro culture model. Methods: AGS and Hep-G2 cell lines, to which H. pylori attaches well are cultured and exposed to H. pylori. The change of the surface microprocesses was analyzed by scanning electron microscopy. Transmission electron microscopy was used to observe the area of contacts. Results: Scanning electron microscopy revealed the decrease in the number of surface microprocesses on both cells by H. pylori contact, and reappeared at 4 hour coculture. Microprocesses of AGS and Hep-G2 cells included microvilli, filopodia, and membrane ruffles. Transmission electron microscoopy showed the actin filament rearrangement in the vicinity of H. pylori contacts. Conclusions: These findings suggest the surface microprocess and cytoskeletal changes may play an important role in the establishment of H. pylori's initial attachment to the gastric epithelial cells. The exact pathophysiological meaning needs to be further investigated. (Korean J Helicobacter Res Prac 2002;2:1-9)

Key Words: Actin, Electron microscopy, Helicobacter pylori, Microprocesses